Anti-PFK-B PFKL Antibody

Product Name

Anti-PFK-B PFKL Antibody

View all PFKL Antibodies

SKU/Catalog Number

A06447

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PFK-B PFKL Antibody catalog # A06447. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PFK-B PFKL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06447)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

6C12

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human PFK-B. at AA rangle: 660-740

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06447 is reactive to PFKL in Human, Mouse, Rat

Applications

A06447 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

85.018kDa

Background of PFKL

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Click image to see more details

Western Blot (WB) analysis of A549 cells using PFK-B Polyclonal antibody.

Click image to see more details

Western Blot (WB) analysis of 3T3 cells using PFK-B Polyclonal antibody.

Gene/Protein Information For PFKL (Source: Uniprot.org, NCBI)

Alternative Names

6-phosphofructokinase, liver type; DKFZp686G1648; DKFZp686L2097; EC 2.7.1; EC 2.7.1.11; FLJ30173; FLJ40909; human liver-type 1-phosphofructokinase, EC 2.7.1.1110Phosphohexokinase; liver-type 1-phosphofructokinase; PFK-B; Phosphofructo-1-kinase isozyme B; Phosphofructokinase 1; phosphofructokinase, liver; phosphohexokinase PFKL ATP-PFK, PFK-B, PFK-L phosphofructokinase, liver type ATP-dependent 6-phosphofructokinase, liver type|6-phosphofructokinase type B|6-phosphofructokinase, liver type|liver-type 1-phosphofructokinase|phosphofructo-1-kinase isozyme B|phosphohexokinase

For more info on PFKL, check out the PFKL Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PFKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PFKL Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis